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Soutien aux Coopérations Universitaires

et Scientifiques Internationales (SCUSI)



“Nouvelle coopération scientifique Lyon-Milan autour des enjeux science-santé en infectiologie”

The Regions Auvergne-Rhône-Alpes and Lombardia are two of the Motors of Europe.
The partnership between 3 groups of the University of Lyon (UdL) and 1 group from the University of Milan Statale (UniMi) aims at strengthening the links between some of the major actors of the European scientific research.
The aim of the SCUSI 2018, coordinated by Prof. A.E. Miele, is to develop a high quality and long-lasting collaboration on the structural dissection of infectious diseases, a challenging multidisciplinary task and one of the priorities of Horizon2020 (“preserving and improving the health and wellbeing of EU citizens”).
Lyon has a long standing history in the molecular characterization of infectious diseases, the translation of the structural knowledge of human pathogens has resulted in better therapeutics and in close partnership of public and private research actors.
Milan has a similar track records in translational research and in the study of structure-function relationships in macromolecules linked to human health.

équipe ASPE (UMR 5246 – ICBMS): Adriana E. MIELE, Sylvie RICARD-BLUM
équipe “structural retrovirology” (UMR 5086 – MMSB): Patrice GOUET, Francesca FIORINI
équipe “biocrystallography and structural biology of therapeutic targets” (UMR 5086 – MMSB): Nushin AGHAJARI, Lionel BALLUT, Sebastien VIOLOT

The SCUSI-TEAM at UniMi:
équipe “structural biology” (Dept. Biosciences): Martino BOOGNESI, Paolo SWUEC, Louise GOURLAY

This new scientific partnership will use an integrative approach based on several molecular biophysics techniques to solve the structures of macromolecular machines from pathogens, and of the complexes between the human host extracellular matrix (ECM) and pathogen proteins.
In particular, biolayer interferometry (BLI), mastered at ASPE, will be used to measure the binding kinetics and affinity of the complexes under study; dynamic light scattering (DLS) and microscale thermophoresis (MST), mastered at MMSB, will be used to asses the homogenity and stability of the samples to be characterised by high resolution cryo-electron microscopy (cryoEM) , at the UniMi facility.
The structural information will be used to start and/or improve the design of immunodiagnostic biomarkers and inhibitors of host-pathogen interactions. A starting point will be provided by screening the chemical library of ICBMS.
This objective is part of a “train through use” strategy which will share science, instrumentation, good laboratory practice and methodological expertise. This partnership will be reinforced by training a new generation of researchers in the advances of structural biology and its application in both basic and translational medical research.