Dr Thierry GRANJON (MCF)

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04 72 43 15 03

2015 - Present : Associate Professor (MCF HDR), Université Claude Bernard Lyon 1
: Habilitation à Diriger les Recherches (HDR), Université Claude Bernard Lyon 1
2005 - 2015
: Assistant Professor (MCF), Université Claude Bernard Lyon 1
2004 : Research Associate, ETH Zürich (Pr. Peter DIMROTH), Switzerland
2001 - 2003 : Postdoctoral studies, Oxford University (Dr. Benjamin BERKS), England
1998-2001: PhD in Biochemistry, Université Claude Bernard Lyon 1 (Prof. Christian VIAL)

1. Marcillat O., Perraut C., Granjon T., Vial C., and Vacheron M. J .  “Cloning, Escherichia coli expression, and phase-transition chromatography-based purification of recombinant rabbit heart mitochondrial creatine kinase.” Protein Expression Purif., 1999, 17 :163168. 

2. Granjon T., Vacheron M. J., Vial C., and Buchet R. “Mitochondrial creatine kinase binding to phospholipids decreases fluidity of membranes and promotes new lipidinduced beta structures as monitored by red edge excitation shift, laurdan fluorescence, and FTIR.” Biochemistry, 2001, 40:6016-6026.  

3. Granjon T., Vacheron M. J., Vial C., and Buchet R. “Structural changes of mitochondrial creatine kinase upon binding of ADP, ATP, or Pi, observed by reactioninduced infrared difference spectra.” Biochemistry, 2001, 40:2988-2994  

4. Granjon T., Vial C., Buchet R., and Vacheron M. J. “Mitochondrial creatine kinase binding to liposomes and vesicle aggregation: effect of cleavage by proteinase K.”  J. Prot. Chem., 2001, 20: 593-599. 

5. De Leeuw E., Granjon T., Porcelli I., Alami M., Carr S. B., Muller M., Sargent F., Palmer T., and Berks B. C. “Oligomeric properties and signal peptide binding by Escherichia coli Tat protein transport complexes.” J Mol Biol., 2002, 322:1135-46. 

6. Granjon T., Vacheron M. J., Buchet R., and Vial C. “Mg-nucleotides induced dissociation of liposome-bound creatine kinase: reversible changes in its secondary structure and in the fluidity of the bilayer.” Mol. Membr. Biol., 2003, 20:163-169.  

7. Dahinden P., Auchli Y., Granjon T., Taralczak M., Wild M., and Dimroth P. “Oxaloacetate decarboxylase of Vibrio cholerae: purification, characterization, and expression of the genes in Escherichia coli.” Arch Microbiol., 2005, 183:121-129.  

8. Vernoux N., Granjon T., Marcillat O., Besson F., and Vial C.  “Interfacial behavior of cytoplasmic and mitochondrial creatine kinase oligomeric states.” Biopolymers, 2006, 81:270-281. 

9. Vernoux N., Maniti O., Besson F., Granjon T., Marcillat O., and Vial C.  “Mitochondrial creatine kinase adsorption to biomimetic membranes: a Langmuir monolayer study.” J. Coll. Int. Sci., 2007, 310:436-445 

10. Leake M. C., Greene N. P., Godun R. M., Granjon T., Buchanan G., Chen S., Berry R. M., Palmer T., and Berks B. C.  “Variable stoichiometry of the TatA component of the twin-arginine protein transport system observed by in vivo single-molecule imaging.” PNAS, 2008, 105:15376-15381

11. Maniti O., Lecompte M. F., Marcillat O., Desbat B., Buchet R., Vial C., and Granjon  T.   “Mitochondrial creatine kinase binding to phospholipid monolayers induces cardiolipin segregation.” Biophys. J., 2009, 96:2428-2438. 

12. Maniti O., Cheniour M., Marcillat O., Vial C. and Granjon T.  “Morphology modifications in negatively charged lipid monolayers upon mitochondrial creatine kinase binding.” Mol. Membr. Biol., 2009, 26: 171-185.       

13. Vernoux N., Maniti O., Marcillat O., Vial C., and Granjon T.  “Mitochondrial creatine kinase interaction with heterogeneous monolayers: Effect on lipid lateral organization.” Biochimie, 2009,  91 : 752-764.  

14. Granjon T., Maniti O., Cheniour M., Lecompte M.F., and Marcillat O.  “Mitochondrial creatine kinase-induced raft-like organization in cardiolipin containing monolayers mimicking the inner mitochondrial membrane.” FEBS J., 2009, 276 : 260-260  

15. Maniti O., Lecompte M.F., Marcillat O. Vial C., and Granjon T.  “Mitochondrial creatine kinase interaction with cardiolipin-containing biomimetic membranes is a two-step process involving adsorption and insertion.” Eur Biophys J., 2010, 39(12):1649-1655  

16. Granjon T., Maniti O., Auchli Y., Dahinden P, Buchet R.,  Marcillat O and Dimroth P.  “Structure-function relations in oxaloacetate decarboxylase complex. Fluorescence and infrared approaches to monitor oxomalonate and Na(+) binding effect. “PLoS One, 2010, 5(6):e10935.               

17. Maniti O., Cheniour M., Lecompte M.F., Marcillat O., Buchet R., Vial C., and Granjon T. “Acyl chain composition determines cardiolipin clustering induced by mitochondrial creatine kinase binding to monolayers.” Biochim Biophys Acta, 2011, 1808(4):11291139. 

18. Francois-Moutal L., Maniti O., Lacombe M.L., Marcillat O. and Granjon T.  “New insights into lipid- Nucleoside Diphosphate Kinase -D interaction mechanism: protein structural changes and membrane reorganization.” Biochim Biophys Acta-Biomembranes, 2012, 1828 (2): 906-915   

19. Jalmar O., François-Moutal, L., García-Sáez A.J., Perry, M., Granjon T., Gonzalvez F., E. Gottlieb, Klösgen B., Ayala-Sanmartin A., Schwille, P. and Petit P.X. “Caspase-8 Binding to Cardiolipin in Giant Unilamellar Vesicles Provides a Functional Docking Platform for Bid”. PLoS One, 2013, 8(2):e55250 

20. François-Moutal, L, Marcillat O., and Granjon T. “Structural comparison of highly similar nucleoside diphosphate kinases: molecular explanation of distinct membrane binding behavior” Biochimie,2014, 105:110-118. doi: 10.1016/j.biochi  

21. Maniti O, Rebaud S, Sarkis J, Jia Y, Zhao J, Marcillat O, Granjon T, Blum L, Li J, Girard-Egrot A. “Carrier-inside-carrier: polyelectrolyte microcapsules as reservoir for drug-loaded liposomes”. J Liposome Res. 2014, 15:1-9 

22. Maniti O., François-Moutal L., Lecompte M.F., Vial C., Lagarde M., Guichardant M., Marcillat O., and Granjon T. ”Protein “amyloid-like” networks at the phospholipid membrane formed by 4-Hydroxynonenal-modified Mitochondrial Creatine Kinase”. Mol. Membr. Biol., 2015, 32(1):1-10 

23. Francois-Moutal L, Ouberai MM, Maniti O, Welland ME, Strzelecka-Kiliszek A, Wos M, Pikula S, Bandorowicz-Pikula J, Marcillat O, and Granjon T. ”Two-Step Membrane Binding of NDPK-B Induces Membrane Fluidity Decrease and Changes in Lipid Lateral Organization and Protein Cluster Formation”. Langmuir. 2016, Dec 6;32(48):12923-12933.

24. Cheniour, M., Gueyrard, D., Goekjian, P.G., Granjon, T., and Marcillat, O. “A convenient and versatile synthesis of Laurdan-like fluorescent membrane probes: characterization of their fluorescence properties” RSC ADVANCES, 2016, Volume: 6, Issue: 7, 5547-5557. 

25. Cheniour M, Brewer J, Bagatolli L, Marcillat O, and Granjon T. « Evidence of proteolipid domain formation in an inner mitochondrial membrane mimicking model ». Biochim Biophys Acta.general subject, 2017, 1861:969-976.

26. Zouaoui J, Trunfio-Sfarghiu AM, Brizuela L, Piednoir A, Maniti O, Munteanu B, Mebarek S, Girard-Egrot A, Landoulsi A, and Granjon T. « Multi-scale mechanical characterization of prostate cancer cell lines: Relevant biological markers to evaluate the cell metastatic potential ». Biochim Biophys Acta.general subject, 2017, 1861(12):3109-3119

Brevet n°  EP19306175.1 1118, ”Fluorescent probe and applications thereof”, 2019.